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KMID : 0545120080180071245
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 7 p.1245 ~ p.1251
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Oxalate Decarboxylase from Agrobacterium tumefaciens C58 is Translocated by a Twin Arginine Translocation System
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Shen Yu-Hu
Liu Rui-Juan
Wang Hai-Qing
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Abstract
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Oxalate decarboxylases (OXDCs) (E.C. 4.1.1.2) are enzymes catalyzing the conversion of oxalate to formate and CO The OXDCs found in fungi and bacteria belong to functionally diverse protein superfamily known as the cupins. Fungi-originated OXDCs are secretory enzymes. However, most bacterial OXDCs are localized in the cytosol, and may be involved in energy metabolism. In Agrobacterium tumefaciens C58, a locus for a putative oxalate decarboxylase is present. In the study reported here, an enzyme was overexpressed in Escherichia coli and showed oxalate carboxylase activity. Computational analysis revealed the A. tumefaciens C58 OXDC contains a signal peptide mediating translocation of the enzyme into the periplasm that was supported by expression of signal-peptideless and full-length versions of the enzyme in A. tumefaciens C58. Further site-directed mutagenesis experiment demonstrated that the A. tumefaciens C58 OXDC is most likely translocated by a twin-arginine translocation (TAT) system.
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KEYWORD
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Oxalate decarboxylase, Agrobacterium tumfaciens, prokaryotic expression, enzymatic activity assay, signal peptide, twin-arginine translocation system
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